Figure 1. Plate containing BL21E.Coli, Ampicillin, and plasmid pGEM-gbr22. At 37 degrees Celsius overnight. Colonies are shown as individual dots.
Figure 2. Control plate containing GBr21 e.coli and ampicillin and grown overnight as well, at 37C, no growth.
Figure3. Fun plate. with no ampicillin present with mucus from a lab member, grown overnight at 37C.
Figure4. Culture of GBR21 in a 125 erlenmeyer flask with ampicillin and LB. Bacteria was successfully grown as purple is expressed in flask.
Figure 5. Pellet solution of GBR21in a 50 mL conical tube. Did centrifuge it but forgot to take picture of dry nonsolution pellet.
Figure6. Elusion 1 & Elusion 2. Gbr22 Protein in solution resuspendend in 1xPBS
Figure7. Elusion 1 trial 1 at 280nm. Absorbance at 280nm (A280).n=2
Figure8. Elusion 1 trial 1 at 574nm. Absorbance at 574nm (A574).n=2
Figure 9. Wet gel. from top to bottom rows were 1. cell lysate 2. soluble fraction. 3. flow through 4. wash 5. elution1 6.did not work 7. elution2 8. cell lysate 9. ladder 10. nothing
Figure 10. dry gel. from bottom to top, rows were 1. cell lysate 2. soluble fraction. 3. flow through 4. wash 5. elution1 6.did not work 7. elution2 8. cell lysate 9. ladder 10. nothing
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References: